Fig. 2

Fig. 2 sop^fixe mutants lack clustered acetylcholine receptors. A,B: The 72 hours postfertilization (hpf) embryos were stained with Alexa-green-conjugated α-bungarotoxin. Clusters of acetylcholine receptor (AChR) were not detected in sop^fixe mutants (B) but are present in wild-type embryos (A). C,D: Wild-type (A) and sop^fixe mutant (B) embryos stained with anti-acetylated tubulin antibody at 72 hpf. Axons of primary motor neurons are present in sop^fixe (D) embryos as in wild-type embryos. E: Fluorescein isothiocyanate (FITC) -dextran marked wild-type muscle cell in a sop^fixe mutant environment shows clustered acetylcholine receptors (arrows) at 24 hpf. Lateral views, anterior to the left, dorsal up. Scale bars = 30 μm in A-D; 12 μm in E.