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Fig. 3 rbmx knockdown in zebrafish. a: Lateral view of wildtype (wt) embryo at 48 hpf. b,c: Lateral view of embryos injected with rbmx morpholino (mo) at 48 hpf. The morphant embryos exhibit a number of phenotypes (arrows) including reduced body size, lack of jaws, delayed reabsorption of the yolk sac, defective somite patterning, curly tail and swollen pericardium. The characteristic defects in brain morphology particularly an inflated tectum and cerebral ventricle are shown in (c). d: In situ hybridization with anti-sense rbmx riboprobe of wildtype (wt) and rbmx morphant (mo) embryos at 72 hpf. e: Partial rescue of morphant (mo+re) phenotype by coinjection of rbmx-morpholino with synthetic, morpholino-resistant rbmx mRNA (lateral view at 72 hpf).