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Fig. 7 Gata4 and Gata6 have redundant functions for liver development, but each gene is essential for liver bud growth. Representative control embryos (A, WT), and embryos injected with the morpholinos specific for Gata4 (B, G4 MO), Gata6 (C, G6 MO), or both (D, G4 +6 MO), are shown following in situ hybridization to detect transcript for the liver differentiation marker transferrin. At 3 dpf, the control embryos show a much enhanced liver growth (arrowhead) compared with either the Gata4 or Gata6 morphants (arrows). However, both morphants consistently show small liver buds. By contrast, injection of both morpholinos results in embryos that completely lack transferrin-positive liver tissue. Views are dorsal, anterior to the left.