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Fig. 4 gbx1 and otx2 expression in Wnt8 gain-of-function experiments. (A) Control embryo stained for gbx1 at 60%. (B-D) Injected embryos with wnt8 RNA 1-5 pg (B), 10-50 pg (C) and 200-400pg (D) stained for gbx1. Overexpression of wnt8 induces ectopic gbx1 expression throughout the epiblast in a dose-dependant way. Overexpression of wnt8 (100 pg) does not induce ectopic gbx1 in Su5402-treated embryos (E) but does so in MZoep embryos (F). (G) Control embryo stained for otx2 at 60%. (H-J) Injected embryos with wnt8 RNA 1-5 pg (H), 10-50 pg (I) and 200-400 pg (J) stained for otx2. Overexpression of wnt8 inhibits otx2 expression. Overexpression of wnt8 (100 pg) in Su5402-treated embryos (K) and in MZoep embryos (L) inhibits otx2 expression. (M-O) wnt8 expression in wild type at 60% (M), in Su5402-treated embryos (N) and in MZoep embryos (O). (A-L) Animal pole views. (M-O) Dorsal views, anterior upwards.