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Fig. 6 The effect of feeding on mtp transcript level in zebrafish as evaluated by real-time quantitative polymerase chain reaction. A: Larvae were fasted for 18 hr and fed or not with dried chicken egg yolk powder for 2 hr. At each developmental stage (dpf, days postfertilization), seven independent pools of more than 30 larvae were used to quantify in duplicate mtp transcript amount. B: Male adult zebrafish were starved for 18 hr and fed or not for 2.5 hr. At each experimental sample, anterior intestine and liver were collected from two animals and pooled. mtp transcript amounts were determined on seven independent samples in duplicate. The indicated values are the mean ± standard deviation of the ratio (microsomal triglyceride transfer protein large subunit copy number/elongation factor 1 α transcript [MTP/EF1α] copy number) × 100. Data were tested for statistical significance by performing a Mann-Whitney test. Asterisks indicate P < 0.05.