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Fig. 5 The effect of feeding on mtp expression in zebrafish larvae as evaluated by whole-mount in situ hybridization. A-I: Larvae were fasted for 18 hr and fed (A,C,D,F,G,I) or not (B,E,H) with dried chicken egg yolk powder for 2 hr. A-I: The 5 days postfertilization (dpf; A-C), 8 dpf (D-F), and 15 dpf (G-I) larvae were then collected and hybridized with sense (A,D,G) or antisense riboprobes (B,C,E,F,H,I). Larvae are shown in lateral view with the anterior part to the left. B,C,E,F,H,I: At each developmental stage, mtp expression in the anterior intestine (ai) dramatically rose in fed larvae (C,F,I) compared with fasted larvae (B,E,H). mtp hybridization signal also appears to be increased in the liver (l). No transcripts could be detected in the posterior intestine (pi). A,D,G: No staining signal has been observed using the control sense probe. Scale bars = 500 μm.