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Fig. 2 RGCs are not required for spread of ath5 expression. (A-C) Expression of ath5 mRNA at 30 hpf (A), 35 hpf (B) and 41 hpf (C) in wild-type fish from a lak/+ incross. At 41 hpf (C), the ath5 wave has progressed into the ventrotemporal retina and has cleared out of the anterior and central regions, but occasionally cells behind the wave front still express ath5 (arrowheads). (D-F) Progression of ath5 wave in lak mutants is indistinguishable from wild-type. Expression of ath5 behind the wave front is reduced in lak mutants, perhaps due to autoregulation of ath5 expression (F; compare with C). Anterior (nasal) is left and dorsal up in all images. Asterisks indicate choroid fissure. Scale bar: 25 µm.