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Fig. S3 Retinoblasts transplanted to forebrain express RGC and photoreceptor markers. Retinal cells from 30 hpf histone 2A:GFP transgenic fish donors were transplanted into 30 hpf non-transgenic host ventral forebrain. At 72 hpf, hosts (n=4) were sectioned and immunostained with zn-8, a marker of RGCs (A-C), or zpr-1, a marker of red/green cone photoreceptors (D-F). Cell type marker analysis was performed on adjacent sections. Although zn-8 is expressed in both brain and RGCs, zpr-1 is specific for photoreceptors. (A,D) GFP donor cells, green. (B,E) Cell type markers, red. (C,F) Overlay showing expression of cell type markers by donor cells (arrowheads). All hosts contained zpr-1+ donor-derived cells in the forebrain, and half the hosts (n=2/4) showed zn-8+ donor-derived cells.