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Fig. 5 olig2 expression is regulated by Hh signals. Lateral trunk views (A-D) and cross-sections through the trunk (E-J) at 22-24 hours postfertilization showing olig2 single in situ hybridization (A-G) and olig2 (blue) and iro3 (red) double in situ hybridization (H-J). A,E,H: Wild-type (wt) embryos. B,F,I: syu mutants. C: smu mutant. D,G,J: Wild-type embryos injected with hh RNA. J: Most iro3 expression has been lost from the intermediate spinal cord, but iro3 is still expressed in motoneurons (example indicated with an arrow; see also Fig. 4I,J). Scale bar = 50 μm in A (applies to A-J).
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