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Fig. 5 tfr1a is required for erythrocyte hemoglobin production, but multiple Tfr family members can compensate for loss of tfr1a in cia. (A-H) Ventral views of the anterior region of o-dianisidine stained embryos at 40 hpf. (A) Uninjected wild type. (B) Uninjected ciaiu089. (C) Wild-type embryo injected with tfr1a MO1 did not exhibit hemoglobinized erythrocytes. (D) Wild-type embryo co-injected with tfr1a MO1 and tfr1a cRNA was partially rescued. (E-H) ciaiu089 embryos injected with cRNA of tfr1a (C), tfr1b (D), mouse tfr1 (E), and mouse tfr2 (F) all exhibited partial rescue of hypochromia.
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