GENOTYPE
Tupfel long fin nacre
Notes
External Notes
The TL-derived TLN
wild type strain carries a mix of molecularly uncharacterized mitfa(nacre)
s170 and mitfa(nacre)
s184 in the background. TL is homozygous for cx41.8(leo)
t1 and lofdt2.
The Baier lab established the TLN line in 2002. Herwig Baier crossed 10 pairs of mitfas170/+(TL) x mitfas184/+(TL) and raised approximately 10 fish from each cross that lacked melanophore pigmentation. Currently, mitfas170 and mitfas184 have not been molecularly characterized, however, non-complementation testing established both strains as mitfa alleles. Thus, this TL-derived strain carries a mix of molecularly uncharacterized mitfa(nacre) s170 and mitfa(nacre) s184 in the background. The line has been propagated by selection for the unpigmented phenotype. Thus, it is unclear if both, or only one of the two mitfa alleles are present in this strain.
In the Baier lab, TLN has been maintained for at least 5 generations by incrossing as many individuals as possible from each generation, and mixing their progeny for the next generation. It is a convenient strain to score for enhancer trap lines with fluorescent reporters.
To avoid bottlenecks, genetic drift, or fixation of unfavorable phenotypes due to inbreeding, TLN can be outcrossed to TL, which gives rise to a pigmented F1 generation. Inbreeding F1 fish and selecting for unpigmented F2 will establish the TLN strain again. (1)
The Baier lab established the TLN line in 2002. Herwig Baier crossed 10 pairs of mitfas170/+(TL) x mitfas184/+(TL) and raised approximately 10 fish from each cross that lacked melanophore pigmentation. Currently, mitfas170 and mitfas184 have not been molecularly characterized, however, non-complementation testing established both strains as mitfa alleles. Thus, this TL-derived strain carries a mix of molecularly uncharacterized mitfa(nacre) s170 and mitfa(nacre) s184 in the background. The line has been propagated by selection for the unpigmented phenotype. Thus, it is unclear if both, or only one of the two mitfa alleles are present in this strain.
In the Baier lab, TLN has been maintained for at least 5 generations by incrossing as many individuals as possible from each generation, and mixing their progeny for the next generation. It is a convenient strain to score for enhancer trap lines with fluorescent reporters.
To avoid bottlenecks, genetic drift, or fixation of unfavorable phenotypes due to inbreeding, TLN can be outcrossed to TL, which gives rise to a pigmented F1 generation. Inbreeding F1 fish and selecting for unpigmented F2 will establish the TLN strain again. (1)