EST
IMAGE:7160305
- ID
- ZDB-EST-080917-129
- Name
- IMAGE:7160305
- Symbol
- IMAGE:7160305
- Previous Names
- None
- Type
- EST
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- GISZF001_ra
- Digest
- Insert Size
- 1800
- Cloning Site
- Sfi A - Sfi B
- Vector Type
- plasmid
- Vector
- pDNR-LIB
- Polymerase
- T3 RNA polymerase
- PCR Amplification
-
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3' - Suppliers
- None
- Quality
- ( Simple to use, intense expression pattern restricted to a few structures )
- Protocol
- Thisse in situ hybridization protocol
- Note
- None
Gene Expression
- Directly Submitted Expression Data
- 6 figures (13 images) from Thisse et al., 2004 [IMAGE:7160305]
Marker Relationships
Sequences
Citations