IMAGE:6896966

ID

ZDB-EST-050506-157

Name

IMAGE:6896966

Symbol

IMAGE:6896966

Previous Names

• FR_3727 (1)

Type

EST

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

GISZF001_ra

Digest

Insert Size

2100

Cloning Site

Sfi A - Sfi B

Vector Type

plasmid

Vector

pDNR-LIB

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'

Suppliers

None

Protocol

Thisse in situ hybridization protocol

Note

None