EST
cb511
- ID
- ZDB-EST-021126-22
- Name
- cb511
- Symbol
- cb511
- Previous Names
- None
- Type
- EST
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- oligo-dT primed segmentation stage
- Digest
- NotI
- Insert Size
- 1200
- Cloning Site
- EcoRI-SalI Digest
- Vector Type
- Plasmid
- Vector
- pSPORT1
- Polymerase
- T3 RNA polymerase
- PCR Amplification
- (replaces the SP6 promoter with a T3 promoter) T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3' T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
- Suppliers
- Quality
- ( Nice strong expression pattern )
- Protocol
- Thisse in situ hybridization protocol
- Note
-
The probe available from ZIRC is a 1200 nt subclone of AF371368 in the pSport vector and should be amplified using PCR.
Gene Expression
- Directly Submitted Expression Data
- 7 figures (49 images) from Thisse et al., 2001 [cb511]
Marker Relationships
Sequences
Citations