EST
cb405
- ID
- ZDB-EST-021024-2
- Name
- cb405
- Symbol
- cb405
- Previous Names
- None
- Type
- EST
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- oligo-dT primed 26-somite stage
- Digest
- PCR amplification
- Insert Size
- 1400
- Cloning Site
- EcoRI-SalI
- Vector Type
- Plasmid
- Vector
- pSPORT1
- Polymerase
- T3 RNA polymerase
- PCR Amplification
-
5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
5' TAA TAC GAC TCA CTA TAG GG 3' - Suppliers
- Quality
- ( Simple to use, intense expression pattern restricted to a few structures )
- Protocol
- Thisse in situ hybridization protocol
- Note
-
Due to the poor incorporation of DIG-NTP by the SP6 RNA polymerase, antisense RNA probes are synthesized from a PCR amplified fragment in which a T3 RNA polymerase replace the SP6 RNA polymerase promoter.
Gene Expression
- Directly Submitted Expression Data
- 5 figures (12 images) from Thisse et al., 2001 [cb405]
Marker Relationships
Sequences
Citations