CRISPR

CRISPR1-rcan3

ID
ZDB-CRISPR-250122-34
Name
CRISPR1-rcan3
Previous Names
None
Target
Sequence
5' - GGACTTGAGTGATTTACCTA - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "CGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
scu103 rcan3
Expression
Gene expression in Wild Types + CRISPR1-rcan3
No data available
Phenotype
Phenotype resulting from CRISPR1-rcan3
No data available
Phenotype of all Fish created by or utilizing CRISPR1-rcan3
Phenotype Fish Conditions Figures
heart decreased functionality, abnormal rcan3scu103/scu103 standard conditions Fig. S5 from Hu et al., 2024
heart contraction irregular rhythm, abnormal rcan3scu103/scu103 standard conditions Fig. S5 from Hu et al., 2024
heart myl7 expression decreased distribution, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
cardiac muscle Z disc morphology, abnormal rcan3scu103/scu103 standard conditions Fig. S5 from Hu et al., 2024
cardiac muscle sarcomere disorganized, abnormal rcan3scu103/scu103 standard conditions Fig. S5 from Hu et al., 2024
heart myl7 expression decreased amount, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
heart notch1b expression decreased amount, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
heart mybpc3 expression decreased distribution, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
heart nkx2.5 expression decreased distribution, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
heart mybpc3 expression decreased amount, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
heart notch1b expression decreased distribution, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
heart contraction decreased rate, abnormal rcan3scu103/scu103 standard conditions Fig. S5 from Hu et al., 2024
heart nkx2.5 expression decreased amount, abnormal rcan3scu103/scu103 standard conditions Fig. 2 from Hu et al., 2024
Citations
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