CRISPR

CRISPR2-furina

ID
ZDB-CRISPR-241122-2
Name
CRISPR2-furina
Previous Names
None
Target
Sequence
5' - GGGCGTCACTGGCAAAATCACGA - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The first three "G"s were added. The PAM site was "CGG" at the 3' end. ZDB-CRISPR-241122-1 and ZDB-CRISPR-241122-2 were designed to be applied together in order to delete a 1.3 kb region in the 3'-UTR of a variant transcript of furina. The deleted region contains the YBE (Ybx1 binding element).
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
No data available
Expression
Gene expression in Wild Types + CRISPR2-furina
No data available
Phenotype
Phenotype resulting from CRISPR2-furina
No data available
Phenotype of all Fish created by or utilizing CRISPR2-furina
Phenotype Fish Conditions Figures
lateral plate mesoderm right side spaw expression mislocalised, abnormal TU + CRISPR1-furina + CRISPR2-furina standard conditions Fig. 8. with image from Nagorska et al., 2023
determination of left/right asymmetry in lateral mesoderm decreased process quality, abnormal TU + CRISPR1-furina + CRISPR2-furina standard conditions Fig. 8. with image from Nagorska et al., 2023
Citations
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.