CRISPR

CRISPR1-furina

ID
ZDB-CRISPR-241122-1
Name
CRISPR1-furina
Previous Names
None
Target
Sequence
5' - GGGTATTCAGACGAACCCCAGA - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The first two "G"s were added. The PAM site was "AGG" at the 3' end. ZDB-CRISPR-241122-1 and ZDB-CRISPR-241122-2 were designed to be applied together in order to delete a 1.3 kb region in the 3'-UTR of a variant transcript of furina. The deleted region contains the YBE (Ybx1 binding element).
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
No data available
Expression
Gene expression in Wild Types + CRISPR1-furina
No data available
Phenotype
Phenotype resulting from CRISPR1-furina
No data available
Phenotype of all Fish created by or utilizing CRISPR1-furina
Phenotype Fish Conditions Figures
lateral plate mesoderm right side spaw expression mislocalised, abnormal TU + CRISPR1-furina + CRISPR2-furina standard conditions Fig. 8. with image from Nagorska et al., 2023
determination of left/right asymmetry in lateral mesoderm decreased process quality, abnormal TU + CRISPR1-furina + CRISPR2-furina standard conditions Fig. 8. with image from Nagorska et al., 2023
Citations
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