CRISPR

CRISPR1-atp6v1ba

ID

ZDB-CRISPR-241104-6

Name

CRISPR1-atp6v1ba

Previous Names

None

Target

atp6v1ba (1)

Sequence

5' - GCACAATCTCTGCGTAACGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
oi9	atp6v1ba

Expression

Gene expression in Wild Types + CRISPR1-atp6v1ba

No data available

Phenotype

Phenotype resulting from CRISPR1-atp6v1ba

No data available

Phenotype of all Fish created by or utilizing CRISPR1-atp6v1ba

Phenotype	Fish	Conditions	Figures
otolith decreased size, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 2 from Ikeuchi et al., 2024
hair cell apical side ab1-tuba labeling decreased amount, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 3 from Ikeuchi et al., 2024
hair cell lysosomal lumen acidification decreased process quality, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 4 from Ikeuchi et al., 2024
whole organism decreased length, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 2 from Ikeuchi et al., 2024
hair cell lysosome decreased acidity, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 4 from Ikeuchi et al., 2024
otic epithelium hair cell has fewer parts of type hair cell cilium, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 3 from Ikeuchi et al., 2024
hair cell macroautophagy increased process quality, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 3 from Ikeuchi et al., 2024
otic sensory epithelium disorganized, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 3 from Ikeuchi et al., 2024
inner ear hair cell ab3-map1lc3b labeling increased amount, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 4 from Ikeuchi et al., 2024
hair cell cilium decreased thickness, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 3 from Ikeuchi et al., 2024
whole organism lethal (sensu genetics), abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 2 from Ikeuchi et al., 2024
otolith formation decreased process quality, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 2 from Ikeuchi et al., 2024
hair cell autophagosome assembly increased process quality, abnormal	atp6v1ba^oi9/oi9	standard conditions	Fig. 4 from Ikeuchi et al., 2024
whole organism regulation of cellular pH decreased process quality, abnormal	atp6v1ba^oi9/oi9; oi10Tg	standard conditions	Fig. 2 from Ikeuchi et al., 2024
whole organism increased acidity, abnormal	atp6v1ba^oi9/oi9; oi10Tg	standard conditions	Fig. 2 from Ikeuchi et al., 2024

Citations