CRISPR

CRISPR3-surf1

ID

ZDB-CRISPR-231108-15

Name

CRISPR3-surf1

Previous Names

None

Target

surf1 (1)

Sequence

5' - CACTCTACAGGCAGTGGGATTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
cri2	surf1

Expression

Gene expression in Wild Types + CRISPR3-surf1

No data available

Phenotype

Phenotype resulting from CRISPR3-surf1

No data available

Phenotype of all Fish created by or utilizing CRISPR3-surf1

Phenotype	Fish	Conditions	Figures
brain opaque, ameliorated	surf1^cri2/cri2	chemical treatment by environment: N-acetyl-L-cysteine, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
brain transparent, abnormal	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
swimming behavior decreased process quality, exacerbated	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 5 from Haroon et al., 2023
swimming behavior decreased process quality, abnormal	surf1^cri2/cri2	standard conditions	Fig. 3, Fig. 5 from Haroon et al., 2023
heart contraction decreased process quality, exacerbated	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 5 from Haroon et al., 2023
glutathione metabolic process decreased process quality, abnormal	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023
heart contraction process quality, ameliorated	surf1^cri2/cri2	chemical treatment by environment: N-acetyl-L-cysteine, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
thigmotaxis process quality, ameliorated	surf1^cri2/cri2	chemical treatment by environment: N-acetyl-L-cysteine, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
reactive oxygen species biosynthetic process rate, ameliorated	surf1^cri2/cri2	chemical treatment by environment: N-acetyl-L-cysteine, chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023
thigmotaxis decreased process quality, exacerbated	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 5 from Haroon et al., 2023
regulation of cytochrome-c oxidase activity decreased process quality, abnormal	surf1^cri2/cri2	standard conditions	Fig. 2 from Haroon et al., 2023
regulation of cytochrome-c oxidase activity decreased process quality, abnormal	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023
reactive oxygen species biosynthetic process rate, ameliorated	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023
whole organism Ab4-cox4 labeling decreased amount, abnormal	surf1^cri2/cri2	standard conditions	Fig. 2 from Haroon et al., 2023
thigmotaxis decreased process quality, abnormal	surf1^cri2/cri2	standard conditions	Fig. 5 from Haroon et al., 2023
heart contraction decreased process quality, abnormal	surf1^cri2/cri2	standard conditions	Fig. 5 from Haroon et al., 2023
swimming behavior decreased process quality, abnormal	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
lactate biosynthetic process increased rate, abnormal	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023
lactate biosynthetic process increased rate, exacerbated	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023
brain transparent, exacerbated	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 5 from Haroon et al., 2023
heart contraction decreased process quality, abnormal	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
swimming behavior process quality, ameliorated	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
glutathione metabolic process process quality, ameliorated	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023
thigmotaxis decreased process quality, abnormal	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
brain opaque, ameliorated	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
swimming behavior process quality, ameliorated	surf1^cri2/cri2	chemical treatment by environment: N-acetyl-L-cysteine, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
thigmotaxis process quality, ameliorated	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
brain transparent, abnormal	surf1^cri2/cri2	standard conditions	Fig. 5 from Haroon et al., 2023
heart contraction process quality, ameliorated	surf1^cri2/cri2	chemical treatment by environment: cysteamine bitartrate, chemical treatment by environment: sodium azide	Fig. 6 from Haroon et al., 2023
reactive oxygen species biosynthetic process increased rate, abnormal	surf1^cri2/cri2	chemical treatment by environment: sodium azide	Fig. 7 from Haroon et al., 2023

Citations