CRISPR

CRISPR15-dhx15

ID

ZDB-CRISPR-231002-1

Name

CRISPR15-dhx15

Previous Names

None

Target

dhx15 (1)

Sequence

5' - GAGGCTGCAGTTGCCCGTCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
idb1	dhx15

Expression

Gene expression in Wild Types + CRISPR15-dhx15

No data available

Phenotype

Phenotype resulting from CRISPR15-dhx15

No data available

Phenotype of all Fish created by or utilizing CRISPR15-dhx15

Phenotype	Fish	Conditions	Figures
intersegmental vein GFP expression spatial pattern, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
whole organism prox1a expression decreased amount, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
post-vent region asymmetrically curved, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
liver RFP expression decreased distribution, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
liver RFP expression decreased amount, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
post-vent region curved, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
pericardium edematous, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024 Fig. 3 from Ribera et al., 2021
post-vent region kinked, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
liver RFP expression absent, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
liver decreased size, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
whole organism hdac3 expression decreased amount, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
whole organism vegfc expression decreased amount, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
eye morphology, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024 Fig. 3 from Ribera et al., 2021
lymphangioblast cord decreased amount, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
posterior cardinal vein GFP expression spatial pattern, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
brain edematous, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
intersegmental vein increased diameter, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
posterior cardinal vein increased diameter, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
whole organism dead, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Fig. 3 from Ribera et al., 2021
whole organism gata6 expression increased amount, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
whole organism ppp1r12a expression decreased amount, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
ventricular system edematous, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024
liver absence of anatomical entity, abnormal	dhx15^idb1/idb1; la116Tg; zf3396Tg	standard conditions	Figure 1 from Portolés et al., 2024

Citations