CRISPR

CRISPR1-ctsk

ID

ZDB-CRISPR-230703-1

Name

CRISPR1-ctsk

Previous Names

None

Target

Sequence

5' - GGGATCATGCGGCTCCTGCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first "G" was added. The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR1-ctsk

No data available

Phenotype

Phenotype resulting from CRISPR1-ctsk

No data available

Phenotype of all Fish created by or utilizing CRISPR1-ctsk

Phenotype	Fish	Conditions	Figures
vertebra bone mineralization increased process quality, abnormal	WT + CRISPR1-ctsk + CRISPR2-ctsk	standard conditions	Fig. 7 from Kague et al., 2021
vertebra increased mass density, abnormal	WT + CRISPR1-ctsk + CRISPR2-ctsk	standard conditions	Fig. 7 from Kague et al., 2021
bone callus protruding out of vertebra, abnormal	WT + CRISPR1-ctsk + CRISPR2-ctsk	standard conditions	Fig. 7 from Kague et al., 2021
vertebral body end plate fibrotic, abnormal	WT + CRISPR1-ctsk + CRISPR2-ctsk	standard conditions	Fig. 7 from Kague et al., 2021
vertebral body end plate bone mineralization increased process quality, abnormal	WT + CRISPR1-ctsk + CRISPR2-ctsk	standard conditions	Fig. 7 from Kague et al., 2021
vertebral body end plate collagen trimer organization quality, abnormal	WT + CRISPR1-ctsk + CRISPR2-ctsk	standard conditions	Fig. 7 from Kague et al., 2021
vertebral body end plate hard, abnormal	WT + CRISPR1-ctsk + CRISPR2-ctsk	standard conditions	Fig. 7 from Kague et al., 2021

Citations