CRISPR

CRISPR1-vrk1

ID

ZDB-CRISPR-230607-3

Name

CRISPR1-vrk1

Previous Names

None

Target

Sequence

5' - CTGTCAGGACTTCTCCAGAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end. This CRISPR also appears to target a processed pseudogene ENSDARG00000091657.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
oi6	vrk1

Expression

Gene expression in Wild Types + CRISPR1-vrk1

No data available

Phenotype

Phenotype resulting from CRISPR1-vrk1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-vrk1

Phenotype	Fish	Conditions	Figures
spermatogenesis occurrence, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022
testis has fewer parts of type sperm, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022
brain size, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon generation of neurons process quality, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
brain nuclear envelope morphology, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
dorsal telencephalon has number of radial glial cell, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
testis size, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022
dorsal telencephalon neural precursor cell proliferation process quality, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
brain nuclear envelope organization process quality, ameliorated	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 3 from Apridita Sebastian et al., 2022
male organism male sterile, abnormal	ankle2^oi5/oi5; vrk1^oi6/+ (AB)	standard conditions	Fig. 4 from Apridita Sebastian et al., 2022

Citations