CRISPR

CRISPR2-appa

ID

ZDB-CRISPR-230526-1

Name

CRISPR2-appa

Previous Names

None

Target

Sequence

5' - AAATGGGAGCCGGATCCGTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3579	appa

Expression

Gene expression in Wild Types + CRISPR2-appa

No data available

Phenotype

Phenotype resulting from CRISPR2-appa

No data available

Phenotype of all Fish created by or utilizing CRISPR2-appa

Phenotype	Fish	Conditions	Figures
whole organism ab2-app labeling decreased amount, abnormal	appa^{zf3579/zf3579}; appb^{zf3355/zf3355} (AB)	standard conditions	Figure 6 from Chebli et al., 2021
whole organism Ab8-app labeling decreased amount, abnormal	appa^{zf3579/zf3579}; appb^{zf3355/zf3355} (AB)	standard conditions	Figure 6 from Chebli et al., 2021
whole organism appa expression decreased amount, abnormal	appa^{zf3579/zf3579}; appb^{zf3355/zf3355} (AB)	standard conditions	Figure 6 from Chebli et al., 2021
whole organism appb expression decreased amount, abnormal	appa^{zf3579/zf3579}; appb^{zf3355/zf3355} (AB)	standard conditions	Figure 6 from Chebli et al., 2021
third ventricle cilium increased length, abnormal	appa^{zf3579/zf3579}; appb^{zf3355/zf3355} (AB)	standard conditions	Figure 7 from Chebli et al., 2021
third ventricle decreased area, abnormal	appa^{zf3579/zf3579}; appb^{zf3355/zf3355} (AB)	standard conditions	Figure 9 from Chebli et al., 2021
third ventricle decreased volume, abnormal	appa^{zf3579/zf3579}; appb^{zf3355/zf3355} (AB)	standard conditions	Figure 9 from Chebli et al., 2021

Citations