CRISPR

CRISPR3-ctcf

ID
ZDB-CRISPR-230509-1
Name
CRISPR3-ctcf
Previous Names
None
Target
Sequence
5' - GGAGTTACACTTGCCCACGC - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The first two "G"s were added.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
upo370 ctcf
Expression
Gene expression in Wild Types + CRISPR3-ctcf
No data available
Phenotype
Phenotype resulting from CRISPR3-ctcf
No data available
Phenotype of all Fish created by or utilizing CRISPR3-ctcf
Phenotype Fish Conditions Figures
embryonic structure hoxd13a expression decreased amount, abnormal ctcfupo370/upo370 standard conditions Fig. 7 with image from Franke et al., 2021
heart edematous, abnormal ctcfupo370/upo370 standard conditions Fig. 1 with image from Franke et al., 2021
brain ptch2 expression increased distribution, abnormal ctcfupo370/upo370 standard conditions Fig. 6 with image from Franke et al., 2021
head decreased size, abnormal ctcfupo370/upo370 standard conditions Fig. 1 with image from Franke et al., 2021
whole organism decreased pigmentation, abnormal ctcfupo370/upo370 standard conditions Fig. 1 with image from Franke et al., 2021
eye decreased size, abnormal ctcfupo370/upo370 standard conditions Fig. 1 with image from Franke et al., 2021
somite ptch2 expression mislocalised, abnormal ctcfupo370/upo370 standard conditions Fig. 6 with image from Franke et al., 2021
pectoral fin bud shha expression absent, abnormal ctcfupo370/upo370 standard conditions Fig. 6 with image from Franke et al., 2021
pectoral fin bud ptch2 expression absent, abnormal ctcfupo370/upo370 standard conditions Fig. 6 with image from Franke et al., 2021
spinal cord ptch2 expression increased distribution, abnormal ctcfupo370/upo370 standard conditions Fig. 6 with image from Franke et al., 2021
pharyngeal arch 3-7 shha expression decreased amount, abnormal ctcfupo370/upo370 standard conditions Fig. 6 with image from Franke et al., 2021
embryonic structure hoxd4a expression decreased amount, abnormal ctcfupo370/upo370 standard conditions Fig. 7 with image from Franke et al., 2021
pharyngeal arch ptch2 expression increased distribution, abnormal ctcfupo370/upo370 standard conditions Fig. 6 with image from Franke et al., 2021
Citations
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