CRISPR

CRISPR2-ift74

ID

ZDB-CRISPR-230324-4

Name

CRISPR2-ift74

Previous Names

None

Target

ift74 (1)

Sequence

5' - TGCCCGGACGGGCCGTCCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ouc2031	ift74

Expression

Gene expression in Wild Types + CRISPR2-ift74

No data available

Phenotype

Phenotype resulting from CRISPR2-ift74

No data available

Phenotype of all Fish created by or utilizing CRISPR2-ift74

Phenotype	Fish	Conditions	Figures
long double cone cell cell body decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 3 from Zhu et al., 2021
anterior crista cilium decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
post-vent region curved, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 1 from Zhu et al., 2021
lateral crista cilium decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
macula cilium decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
retinal rod cell rod photoreceptor outer segment decreased amount, abnormal	ift74^{ouc2031/ouc2031}	control	Figure 6 from Zhu et al., 2021
neuromast cilium decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
long double cone cell decreased amount, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 3 from Zhu et al., 2021
pronephric duct cilium decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
macula cilium ab2-tub-glyc labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
short double cone cell cell body decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 3 from Zhu et al., 2021
pronephric duct cilium ab2-tub-glyc labeling spatial pattern, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
short double cone cell zpr-1 labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 3 from Zhu et al., 2021
posterior crista cilium ab2-tub-glyc labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
pronephric duct cilium ab2-tub-glyc labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
pronephric duct cilium disorganized, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
short double cone cell decreased amount, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 3 from Zhu et al., 2021
lateral crista cilium ab2-tub-glyc labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
olfactory pit cilium decreased amount, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
anterior crista cilium ab2-tub-glyc labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
olfactory pit cilium ab2-tub-glyc labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
long double cone cell zpr-1 labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 3 from Zhu et al., 2021
retinal rod cell rod photoreceptor outer segment zpr-3 labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	control	Figure 6 from Zhu et al., 2021
posterior crista cilium decreased length, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
spinal cord cilium ab2-tub-glyc labeling absent, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
spinal cord cilium absence of anatomical entity, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
eye photoreceptor cell photoreceptor connecting cilium decreased amount, abnormal	ift74^{ouc2031/ouc2031}	control	Figure 4 , Figure 6 from Zhu et al., 2021
neuromast cilium ab2-tub-glyc labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 2 from Zhu et al., 2021
eye photoreceptor cell photoreceptor connecting cilium Ab15-tuba labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031}	standard conditions	Figure 4 , Figure 6 from Zhu et al., 2021
eye photoreceptor cell photoreceptor connecting cilium decreased amount, abnormal	ift74^{ouc2031/ouc2031} + MO3-ift74	control	Figure 6 from Zhu et al., 2021
eye photoreceptor cell photoreceptor connecting cilium Ab15-tuba labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031} + MO3-ift74	control	Figure 6 from Zhu et al., 2021
retinal rod cell rod photoreceptor outer segment zpr-3 labeling decreased distribution, abnormal	ift74^{ouc2031/ouc2031} + MO3-ift74	control	Figure 6 from Zhu et al., 2021
retinal rod cell rod photoreceptor outer segment decreased amount, abnormal	ift74^{ouc2031/ouc2031} + MO3-ift74	control	Figure 6 from Zhu et al., 2021
eye photoreceptor cell photoreceptor connecting cilium decreased length, abnormal	ift74^{ouc2031/ouc2031} + MO3-ift74	control	Figure 6 from Zhu et al., 2021
post-vent region curved, abnormal	ift74^ouc2031/+; ift74^ouc2030/+	standard conditions	Figure 1 from Zhu et al., 2021
eye photoreceptor cell opsin transport decreased rate of continuous process, abnormal	ift74^{ouc2031/ouc2031}; ouc2001Tg	heat shock	Figure 5 from Zhu et al., 2021
eye photoreceptor cell photoreceptor inner segment GFP expression mislocalised, abnormal	ift74^{ouc2031/ouc2031}; ouc2001Tg	heat shock	Figure 5 from Zhu et al., 2021
eye photoreceptor cell cell body GFP expression increased amount, abnormal	ift74^{ouc2031/ouc2031}; ouc2001Tg	heat shock	Figure 5 from Zhu et al., 2021

Citations