CRISPR

CRISPR1-tfe3a

ID
ZDB-CRISPR-230227-6
Name
CRISPR1-tfe3a
Previous Names
None
Target
Sequence
5' - GGAGTCAGCCTATTACGCCAAG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The first "G" was added. The PAM site was "TGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
st124 tfe3a
Expression
Gene expression in Wild Types + CRISPR1-tfe3a
No data available
Phenotype
Phenotype resulting from CRISPR1-tfe3a
No data available
Phenotype of all Fish created by or utilizing CRISPR1-tfe3a
Phenotype Fish Conditions Figures
midbrain microglial cell amount, ameliorated rragast77/st77; tfe3ast124/+ standard conditions Fig. 4 with image from Iyer et al., 2022
microglial cell apoeb expression amount, ameliorated rragast77/st77; tfe3ast124/st124 standard conditions Fig. 5 with image from Iyer et al., 2022
midbrain microglial cell amount, ameliorated rragast77/st77; tfe3ast124/st124 standard conditions Fig. 4 with image from Iyer et al., 2022
microglial cell lysosomal lumen acidification normal occurrence, ameliorated rragast77/st77; tfe3ast124/st124 standard conditions Fig. 5 with image from Iyer et al., 2022
midbrain microglial cell normal amount, ameliorated rragast77/st77; tfe3ast124/+; tfe3bst125/+; tfebst120/+ standard conditions Fig. 4 with image from Iyer et al., 2022
Citations
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