CRISPR

CRISPR2-flcn

ID
ZDB-CRISPR-230227-10
Name
CRISPR2-flcn
Previous Names
None
Target
Sequence
5' - GGCTGGGATGGTGCTGAAGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
st127 flcn
Expression
Gene expression in Wild Types + CRISPR2-flcn
No data available
Phenotype
Phenotype resulting from CRISPR2-flcn
No data available
Phenotype of all Fish created by or utilizing CRISPR2-flcn
Phenotype Fish Conditions Figures
microglial cell lysosomal lumen acidification increased occurrence, abnormal flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell apoeb expression decreased amount, abnormal flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell EGFP expression spatial pattern, abnormal flcnst127/st127; gl22Tg/gl22Tg standard conditions Fig. 5 with image from Iyer et al., 2022
macrophage EGFP expression spatial pattern, abnormal flcnst127/st127; gl22Tg/gl22Tg standard conditions Fig. 5 with image from Iyer et al., 2022
midbrain microglial cell amount, ameliorated tfe3bst125/+; tfebst120/+; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
midbrain microglial cell amount, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell lysosomal lumen acidification normal occurrence, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell apoeb expression amount, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127 standard conditions Fig. 5 with image from Iyer et al., 2022
macrophage EGFP expression spatial pattern, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127; gl22Tg/gl22Tg standard conditions Fig. 5 with image from Iyer et al., 2022
microglial cell EGFP expression spatial pattern, ameliorated tfe3bst125/st125; tfebst120/st120; flcnst127/st127; gl22Tg/gl22Tg standard conditions Fig. 5 with image from Iyer et al., 2022
Citations
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