CRISPR

CRISPR1-srrm3

ID

ZDB-CRISPR-230125-1

Name

CRISPR1-srrm3

Previous Names

None

Target

srrm3 (1)

Sequence

5' - GGGAATAACTGCGTGAGCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
crg3	srrm3
crg5	srrm3

Expression

Gene expression in Wild Types + CRISPR1-srrm3

No data available

Phenotype

Phenotype resulting from CRISPR1-srrm3

No data available

Phenotype of all Fish created by or utilizing CRISPR1-srrm3

Phenotype	Fish	Conditions	Figures
retina decreased functionality, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
visual system decreased functionality, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 , Fig. S10 from Ciampi et al., 2022
eye photoreceptor cell photoreceptor outer segment disorganized, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retina alternative mRNA splicing, via spliceosome process quality, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 6 from Ciampi et al., 2022
retinal cone cell photoreceptor outer segment disorganized, abnormal	srrm3^crg3/crg3	standard conditions	Fig. S11 from Ciampi et al., 2022
retina mitochondrion decreased size, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retina mitochondrion decreased area, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retina mitochondrion fragmented, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
eye photoreceptor cell photoreceptor outer segment decreased length, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
whole organism dead, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 4 from Ciampi et al., 2022
retinal rod cell photoreceptor outer segment disorganized, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retina mitochondrion decreased amount, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retinal outer nuclear layer absence due to degeneration, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retinal outer nuclear layer decreased thickness, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retinal photoreceptor layer apoptotic process increased occurrence, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
retinal rod cell photoreceptor outer segment decreased length, abnormal	srrm3^crg3/crg3	standard conditions	Fig. 5 from Ciampi et al., 2022
visual system decreased functionality, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
eye photoreceptor cell photoreceptor outer segment disorganized, abnormal	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
retina decreased functionality, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
retina mitochondrion fragmented, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
eye photoreceptor cell photoreceptor outer segment decreased length, abnormal	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
whole organism dead, abnormal	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 4 from Ciampi et al., 2022
retina mitochondrion decreased size, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022
retina mitochondrion decreased area, exacerbated	srrm3^crg3/crg3; srrm4^crg4/crg4	standard conditions	Fig. 5 from Ciampi et al., 2022

Citations