CRISPR

CRISPR2-bbs1

ID

ZDB-CRISPR-221104-4

Name

CRISPR2-bbs1

Previous Names

None

Target

bbs1 (1)

Sequence

5' - GGACGTCTGGTGTCAGGCCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ka742	bbs1

Expression

Gene expression in Wild Types + CRISPR2-bbs1

No data available

Phenotype

Phenotype resulting from CRISPR2-bbs1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-bbs1

Phenotype	Fish	Conditions	Figures
photoreceptor outer segment layer bbs4 expression decreased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 5 from Masek et al., 2022
axis kinked, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 1 from Masek et al., 2022
axis kinked, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 1 from Masek et al., 2022
axis kinked, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 1 from Masek et al., 2022
photoreceptor outer segment layer bbs5 expression absent, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 5 from Masek et al., 2022
photoreceptor outer segment layer bbs2 expression absent, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 5 from Masek et al., 2022
axis curved, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 1 from Masek et al., 2022
axis curved, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 1 from Masek et al., 2022
photoreceptor outer segment layer bbs9 expression decreased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 5 from Masek et al., 2022
photoreceptor outer segment layer bbs1 expression absent, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 5 from Masek et al., 2022
photoreceptor outer segment layer morphology, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 5 , Fig. 6 from Masek et al., 2022
photoreceptor outer segment layer shortened, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 3 from Masek et al., 2022
photoreceptor outer segment layer cholesterol increased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 6 from Masek et al., 2022
photoreceptor outer segment layer photoreceptor disc membrane disorganized, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 3 from Masek et al., 2022
photoreceptor outer segment layer photoreceptor disc membrane disorganized, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 6 from Masek et al., 2022
eye bbs1 expression decreased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 4 from Masek et al., 2022
axis curved, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 1 from Masek et al., 2022
photoreceptor outer segment layer phosphatidylinositol 38:5 increased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 6 from Masek et al., 2022
photoreceptor outer segment layer cholesterol increased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 6 from Masek et al., 2022
photoreceptor outer segment layer shape, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 3 from Masek et al., 2022
photoreceptor outer segment layer cholesterol increased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 6 from Masek et al., 2022
retinal pigmented epithelium inclusion body increased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 3 from Masek et al., 2022
photoreceptor outer segment layer bbs7 expression decreased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 5 from Masek et al., 2022
photoreceptor outer segment layer phospholipid increased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 6 from Masek et al., 2022
photoreceptor outer segment layer vesicle increased amount, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 6 from Masek et al., 2022
eye detection of light stimulus involved in visual perception decreased process quality, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 2 from Masek et al., 2022
eye detection of light stimulus involved in visual perception decreased process quality, abnormal	bbs1^ka742/ka742 (AB)	standard conditions	Fig. 2 , Fig. 3 from Masek et al., 2022

Citations