CRISPR

CRISPR2-dlx5a

ID
ZDB-CRISPR-221017-10
Name
CRISPR2-dlx5a
Previous Names
None
Target
Sequence
5' - GGCTCTCGATATAATGGCAT - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505 dlx5a, dlx6a, en.inter56, nccr.dlx5i6
Df(19:nccr.dlx5i6,en.inter56)ot504 en.inter56, nccr.dlx5i6
ot502 dlx5a
Expression
Gene expression in Wild Types + CRISPR2-dlx5a
No data available
Phenotype
Phenotype resulting from CRISPR2-dlx5a
No data available
Phenotype of all Fish created by or utilizing CRISPR2-dlx5a
Phenotype Fish Conditions Figures
brain dlx6a expression decreased amount, abnormal dlx5aot502/ot502 standard conditions Fig. 5 with image from Yu et al., 2021
ceratohyal cartilage malformed, abnormal dlx5aot502/ot502 standard conditions Figure 1 with image from Yu et al., 2023
palatoquadrate cartilage malformed, abnormal dlx5aot502/ot502 standard conditions Figure 1 with image,  Figure 2 with image from Yu et al., 2023
diencephalon dlx2a expression increased amount, abnormal dlx5aot502/ot502 standard conditions Fig. 3 with image from Yu et al., 2021
Meckel's cartilage malformed, abnormal dlx5aot502/ot502 standard conditions Figure 1 with image,  Figure 2 with image from Yu et al., 2023
pharyngeal arch 3-7 dlx2a expression increased amount, abnormal dlx5aot502/ot502 standard conditions Fig. 3 with image from Yu et al., 2021
telencephalon dlx2a expression increased amount, abnormal dlx5aot502/ot502 standard conditions Fig. 3 with image from Yu et al., 2021
Meckel's cartilage col11a2 expression increased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 5 with image from Yu et al., 2023
head fzd7a expression increased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 6 with image from Yu et al., 2023
palatoquadrate cartilage malformed, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 1 with image,  Figure 2 with image from Yu et al., 2023
neural plate foxd3 expression spatial pattern, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 3 with image from Yu et al., 2023
pharyngeal arch neural crest cell sox9a expression increased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 5 with image from Yu et al., 2023
head wnt5b expression increased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 6 with image from Yu et al., 2023
head wnt5b expression decreased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 6 with image from Yu et al., 2023
chondrocyte microtubule organizing center misaligned with axis, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 7 with image from Yu et al., 2023
Meckel's cartilage malformed, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 1 with image,  Figure 2 with image,  Figure 7 with image from Yu et al., 2023
ethmoid cartilage col11a2 expression increased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 5 with image from Yu et al., 2023
head ror2 expression increased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 6 with image from Yu et al., 2023
head wls expression increased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 6 with image from Yu et al., 2023
neural crest cell division increased frequency, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 4 with image from Yu et al., 2023
head fzd7a expression decreased amount, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 6 with image from Yu et al., 2023
chondrocyte microtubule organizing center polar polarity, abnormal Df(19:dlx5a,nccr.dlx5i6,en.inter56,dlx6a)ot505/ot505 standard conditions Figure 7 with image from Yu et al., 2023
palatoquadrate cartilage malformed, abnormal Df(19:nccr.dlx5i6,en.inter56)ot504/ot504 standard conditions Figure 1 with image,  Figure 2 with image from Yu et al., 2023
ceratohyal cartilage malformed, abnormal Df(19:nccr.dlx5i6,en.inter56)ot504/ot504 standard conditions Figure 1 with image from Yu et al., 2023
Meckel's cartilage malformed, abnormal Df(19:nccr.dlx5i6,en.inter56)ot504/ot504 standard conditions Figure 1 with image from Yu et al., 2023
Citations
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