CRISPR

CRISPR4-tet2

ID

ZDB-CRISPR-220126-5

Name

CRISPR4-tet2

Previous Names

None

Target

tet2 (1)

Sequence

5' - CCCAAACATAACAAGGCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hsi12	tet2

Expression

Gene expression in Wild Types + CRISPR4-tet2

No data available

Phenotype

Phenotype resulting from CRISPR4-tet2

No data available

Phenotype of all Fish created by or utilizing CRISPR4-tet2

Phenotype	Fish	Conditions	Figures
erythrocyte differentiation decreased process quality, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 3 from Rajan et al., 2021
erythroid progenitor cell decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 3 from Rajan et al., 2021
myeloid lineage restricted progenitor cell decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
whole organism hbbe1.1 expression decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 3 from Rajan et al., 2021
neutrophilic promyelocyte cebp1 expression increased distribution, abnormal	tet2^hsi12/hsi12	chemical treatment by injection: ManNAz-DBCO-Pam3CSK4	Fig. 7 from Rajan et al., 2021
whole organism hbae1.1 expression decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 3 from Rajan et al., 2021
myeloid cell spi1b expression decreased distribution, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
myeloid lineage restricted progenitor cell spi1b expression decreased distribution, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
myeloid cell cpa5 expression decreased distribution, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
head kidney myeloid cell dysplastic, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 4 from Rajan et al., 2021
whole organism cdkn1a expression increased amount, abnormal	tet2^hsi12/hsi12	chemical treatment by environment: camptothecin	Fig. 6 from Rajan et al., 2021
myeloblast cebp1 expression increased distribution, abnormal	tet2^hsi12/hsi12	chemical treatment by injection: ManNAz-DBCO-Pam3CSK4	Fig. 7 from Rajan et al., 2021
neutrophilic myelocyte cebp1 expression increased distribution, abnormal	tet2^hsi12/hsi12	chemical treatment by injection: ManNAz-DBCO-Pam3CSK4	Fig. 7 from Rajan et al., 2021
myeloid cell differentiation decreased process quality, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
neutrophil mpx expression decreased distribution, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
erythroid progenitor cell gata1a expression decreased distribution, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 3 from Rajan et al., 2021
whole organism ab11-tp53 labeling increased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 6 from Rajan et al., 2021
whole organism decreased length, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 1 from Rajan et al., 2021
head kidney erythroid lineage cell dysplastic, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 4 from Rajan et al., 2021
head kidney hematopoietic cell dysplastic, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 4 from Rajan et al., 2021
myeloid cell decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
leukocyte lcp1 expression decreased distribution, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
whole organism gata1a expression decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 3 from Rajan et al., 2021
leukocyte decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021
whole organism bbc3 expression increased amount, abnormal	tet2^hsi12/hsi12	chemical treatment by environment: camptothecin	Fig. 6 from Rajan et al., 2021
whole organism hbae3 expression decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 3 from Rajan et al., 2021
neutrophil decreased amount, abnormal	tet2^hsi12/hsi12	standard conditions	Fig. 2 from Rajan et al., 2021

Citations