CRISPR

CRISPR1-dcst2

ID

ZDB-CRISPR-220111-3

Name

CRISPR1-dcst2

Previous Names

None

Target

Sequence

5' - GGATAAGATCAAGGAAATGGCG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
vbc15	dcst2

Expression

Gene expression in Wild Types + CRISPR1-dcst2

No data available

Phenotype

Phenotype resulting from CRISPR1-dcst2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-dcst2

Phenotype	Fish	Conditions	Figures
sperm dcst1 expression absent, abnormal	dcst2^vbc15/vbc15 (AB/TL)	standard conditions	Fig. 5 from Noda et al., 2022
male organism male sterile, abnormal	dcst2^vbc15/vbc15 (AB/TL)	standard conditions	Fig. 5 from Noda et al., 2022
sperm sperm head plasma membrane dcst2 expression decreased distribution, abnormal	dcst2^vbc15/vbc15 (AB/TL)	standard conditions	Fig. 5 from Binner et al., 2022
sperm sperm head plasma membrane dcst2 expression spatial pattern, abnormal	dcst2^vbc15/vbc15 (AB/TL)	standard conditions	Fig. 5 from Binner et al., 2022
sperm dcst2 expression absent, abnormal	dcst2^vbc15/vbc15 (AB/TL)	standard conditions	Fig. 5 from Noda et al., 2022
sperm dcst2 expression decreased amount, abnormal	dcst2^vbc15/vbc15 (AB/TL)	standard conditions	Fig. 5, Fig. S2 from Binner et al., 2022
sperm sperm head plasma membrane dcst2 expression absent, abnormal	dcst2^vbc15/vbc15 (AB/TL)	standard conditions	Fig. 5 from Noda et al., 2022

Citations