CRISPR

CRISPR1-dpysl2b

ID
ZDB-CRISPR-220104-1
Name
CRISPR1-dpysl2b
Previous Names
None
Target
Sequence
5' - GGAGAAAATCTAATAGTGCC - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "CGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
wa21 dpysl2b
Expression
Gene expression in Wild Types + CRISPR1-dpysl2b
No data available
Phenotype
Phenotype resulting from CRISPR1-dpysl2b
No data available
Phenotype of all Fish created by or utilizing CRISPR1-dpysl2b
Phenotype Fish Conditions Figures
anterior commissure axon decreased distribution, abnormal dpysl2bwa21/wa21 (RW) standard conditions Fig. 3,  Fig. 5 from Guo et al., 2022
anterior commissure axon ab1-tuba labeling spatial pattern, abnormal dpysl2bwa21/wa21 (RW) standard conditions Fig. 3,  Fig. 5 from Guo et al., 2022
postoptic commissure axon ab1-tuba labeling spatial pattern, abnormal dpysl2bwa21/wa21 (RW) standard conditions Fig. 3,  Fig. 5 from Guo et al., 2022
postoptic commissure axon decreased distribution, abnormal dpysl2bwa21/wa21 (RW) standard conditions Fig. 3,  Fig. 5 from Guo et al., 2022
branchiomotor neuron neuron migration disrupted, abnormal dpysl2bwa21/wa21; rw0Tg standard conditions Fig. 2 from Fiallos-Oliveros et al., 2020
rhombomere 6 branchiomotor neuron displaced to rhombomere 4, abnormal dpysl2bwa21/wa21; rw0Tg standard conditions Fig. 2 from Fiallos-Oliveros et al., 2020
rhombomere 6 branchiomotor neuron displaced to rhombomere 5, abnormal dpysl2bwa21/wa21; rw0Tg standard conditions Fig. 2 from Fiallos-Oliveros et al., 2020
anterior commissure axon spatial pattern, exacerbated dpysl2bwa21/wa21 + MO2-dpysl3 (RW) standard conditions Fig. 5 from Guo et al., 2022
anterior commissure axon ab1-tuba labeling spatial pattern, abnormal dpysl2bwa21/wa21 + MO2-dpysl3 (RW) control Fig. 5 from Guo et al., 2022
postoptic commissure axon ab1-tuba labeling spatial pattern, abnormal dpysl2bwa21/wa21 + MO2-dpysl3 (RW) control Fig. 5 from Guo et al., 2022
postoptic commissure axon spatial pattern, exacerbated dpysl2bwa21/wa21 + MO2-dpysl3 (RW) standard conditions Fig. 5 from Guo et al., 2022
anterior commissure axon ab1-tuba labeling spatial pattern, abnormal dpysl2bwa21/+; dpysl3wa41/+ (RW) standard conditions Fig. 5 from Guo et al., 2022
postoptic commissure axon decreased distribution, abnormal dpysl2bwa21/+; dpysl3wa41/+ (RW) standard conditions Fig. 5 from Guo et al., 2022
postoptic commissure axon ab1-tuba labeling spatial pattern, abnormal dpysl2bwa21/+; dpysl3wa41/+ (RW) standard conditions Fig. 5 from Guo et al., 2022
anterior commissure axon decreased distribution, abnormal dpysl2bwa21/+; dpysl3wa41/+ (RW) standard conditions Fig. 5 from Guo et al., 2022
Citations
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.