CRISPR

CRISPR3-kif7

ID

ZDB-CRISPR-210719-3

Name

CRISPR3-kif7

Previous Names

None

Target

Sequence

5' - GGGGAGCGGAGGGTCACCCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first two "G"s were added. The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR3-kif7

No data available

Phenotype

Phenotype resulting from CRISPR3-kif7

Phenotype	Fish	Figures
determination of heart left/right asymmetry process quality, abnormal	f1Tg + CRISPR3-kif7	Fig. 2 from Liang et al., 2020
determination of left/right symmetry process quality, abnormal	AB + CRISPR3-kif7	Fig. 4 from Liang et al., 2020
determination of liver left/right asymmetry disrupted, abnormal	AB + CRISPR3-kif7	Fig. 3 from Liang et al., 2020
heart position, abnormal	f1Tg + CRISPR3-kif7	Fig. 2 from Liang et al., 2020
heart looping process quality, abnormal	f1Tg + CRISPR3-kif7	Fig. 2 from Liang et al., 2020
liver mislocalised, abnormal	AB + CRISPR3-kif7	Fig. 3 from Liang et al., 2020

Phenotype of all Fish created by or utilizing CRISPR3-kif7

Phenotype	Fish	Conditions	Figures
determination of left/right symmetry process quality, abnormal	AB + CRISPR3-kif7	standard conditions	Fig. 4 from Liang et al., 2020
determination of liver left/right asymmetry disrupted, abnormal	AB + CRISPR3-kif7	standard conditions	Fig. 3 from Liang et al., 2020
liver mislocalised, abnormal	AB + CRISPR3-kif7	standard conditions	Fig. 3 from Liang et al., 2020
heart looping process quality, abnormal	f1Tg + CRISPR3-kif7	standard conditions	Fig. 2 from Liang et al., 2020
heart position, abnormal	f1Tg + CRISPR3-kif7	standard conditions	Fig. 2 from Liang et al., 2020
determination of heart left/right asymmetry process quality, abnormal	f1Tg + CRISPR3-kif7	standard conditions	Fig. 2 from Liang et al., 2020

Citations