CRISPR

CRISPR1-sdhb

ID

ZDB-CRISPR-210716-2

Name

CRISPR1-sdhb

Previous Names

None

Target

sdhb (1)

Sequence

5' - GTTCACCGGCCCGCCGTCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
rmc200	sdhb

Expression

Gene expression in Wild Types + CRISPR1-sdhb

No data available

Phenotype

Phenotype resulting from CRISPR1-sdhb

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sdhb

Phenotype	Fish	Conditions	Figures
whole organism reactive oxygen species increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Figure 1 from Dona et al., 2021
swim bladder absent, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 4 from Dona et al., 2020
locomotory behavior process quality, abnormal	sdhb^{rmc200/rmc200} (AB)	altered light dark cycle	Fig. 5 from Dona et al., 2020
startle response decreased process quality, abnormal	sdhb^{rmc200/rmc200} (AB)	control	Figure 5 from Dona et al., 2021
intestinal mucosa morphology, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. S3 from Dona et al., 2020
whole organism lactate increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
mitochondrial electron transport, succinate to ubiquinone process quality, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism succinate increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism asparagine decreased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism dead, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 3 from Dona et al., 2020
whole organism aspartate decreased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 6 from Dona et al., 2020
whole organism decreased life span, exacerbated	sdhb^{rmc200/rmc200} (AB)	chemical treatment by environment: L-ascorbic acid	Figure 3 from Dona et al., 2021
swim bladder decreased area, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 4 from Dona et al., 2020
swim bladder uninflated, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 4 from Dona et al., 2020
heart decreased functionality, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. 5 from Dona et al., 2020
whole organism decreased life span, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Figure 3 from Dona et al., 2021 Fig. 3 from Dona et al., 2020
startle response decreased process quality, exacerbated	sdhb^{rmc200/rmc200} (AB)	chemical treatment by environment: L-ascorbic acid	Figure 5 from Dona et al., 2021
goblet cell increased amount, abnormal	sdhb^{rmc200/rmc200} (AB)	standard conditions	Fig. S3 from Dona et al., 2020
startle response decreased process quality, abnormal	sdhb^rmc200/+ (AB)	chemical treatment by environment: L-ascorbic acid	Figure 5 from Dona et al., 2021
swim bladder decreased area, abnormal	sdhb^rmc200/+ (AB)	standard conditions	Fig. 4 from Dona et al., 2020

Citations