CRISPR

CRISPR3-cftr

ID

ZDB-CRISPR-210325-4

Name

CRISPR3-cftr

Previous Names

None

Target

Sequence

5' - AATCGTCAACCCTCTTGGGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sh540	cftr

Expression

Gene expression in Wild Types + CRISPR3-cftr

No data available

Phenotype

Phenotype resulting from CRISPR3-cftr

No data available

Phenotype of all Fish created by or utilizing CRISPR3-cftr

Phenotype	Fish	Conditions	Figures
caudal fin fin regeneration decreased occurrence, abnormal	cftr^sh540/sh540	amputation: caudal fin	Figure 4 from Bernut et al., 2020
caudal fin hydrogen peroxide increased amount, exacerbated	cftr^sh540/sh540	amputation: caudal fin	Figure 2 from Bernut et al., 2020
caudal fin neutrophil increased amount, ameliorated	cftr^sh540/sh540; i114Tg	chemical treatment by environment: dibenziodolium chloride, amputation: caudal fin	Figure 2 from Bernut et al., 2020
caudal fin neutrophil increased amount, abnormal	cftr^sh540/sh540; i114Tg	chemical treatment by injection: N-formyl-L-methionyl-L-leucyl-L-phenylalanine, amputation: caudal fin	Figure 1 from Bernut et al., 2020
caudal fin neutrophil increased amount, abnormal	cftr^sh540/sh540; i114Tg	amputation: caudal fin	Figure 2 , Figure 5 from Bernut et al., 2020
caudal fin neutrophil increased amount, exacerbated	cftr^sh540/sh540; i114Tg	chemical treatment by environment: hydrogen peroxide, amputation: caudal fin	Figure 2 from Bernut et al., 2020
caudal fin neutrophil increased amount, abnormal	cftr^sh540/sh540; i114Tg/i114Tg	amputation: caudal fin	Figure 1 from Bernut et al., 2020
innate immune response disrupted, abnormal	cftr^sh540/sh540; i114Tg/i114Tg	amputation: caudal fin	Figure 1 from Bernut et al., 2020

Citations