CRISPR

CRISPR1-p3h1

ID

ZDB-CRISPR-210201-4

Name

CRISPR1-p3h1

Previous Names

None

Target

p3h1 (1)

Sequence

5' - GGAGACCGATTTCAAGGACCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
upv2	p3h1

Expression

Gene expression in Wild Types + CRISPR1-p3h1

No data available

Phenotype

Phenotype resulting from CRISPR1-p3h1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-p3h1

Phenotype	Fish	Conditions	Figures
notochord bone mineralization delayed, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 3 from Tonelli et al., 2020
osteoblast endoplasmic reticulum cisternal network increased size, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 6 from Tonelli et al., 2020
vertebra misaligned with vertebra, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 4 from Tonelli et al., 2020
whole organism decreased length, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 3 from Tonelli et al., 2020
vertebra antero-posteriorly flattened, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 4 from Tonelli et al., 2020
vertebral column antero-posteriorly flattened, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 4 from Tonelli et al., 2020
vertebral body end plate malformed, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 7 from Tonelli et al., 2020
vertebral column deformed, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 4 from Tonelli et al., 2020
vertebra bone tissue decreased volume, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 4 from Tonelli et al., 2020
ceratohyal bone bone mineralization delayed, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 3 from Tonelli et al., 2020
vertebra decreased diameter, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 4 from Tonelli et al., 2020
notochord keratinous, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 7 from Tonelli et al., 2020
chondroblast endoplasmic reticulum cisternal network increased size, abnormal	p3h1^upv2/upv2	standard conditions	Fig. 6 from Tonelli et al., 2020
whole organism decreased length, abnormal	p3h1^upv2/+	standard conditions	Fig. 3 from Tonelli et al., 2020

Citations