CRISPR

CRISPR2-grin1a

ID

ZDB-CRISPR-201201-1

Name

CRISPR2-grin1a

Previous Names

None

Target

grin1a (1)

Sequence

5' - AGCATCCTATACTGCCAACC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sbu90	grin1a
sbu91	grin1a
sbu92	grin1a

Expression

Gene expression in Wild Types + CRISPR2-grin1a

No data available

Phenotype

Phenotype resulting from CRISPR2-grin1a

No data available

Phenotype of all Fish created by or utilizing CRISPR2-grin1a

Phenotype	Fish	Conditions	Figures
eating behavior decreased occurrence, abnormal	grin1a^sbu90/sbu90	standard conditions	Fig. 6 from Zoodsma et al., 2020
whole organism decreased length, abnormal	grin1a^sbu90/sbu90	standard conditions	Fig. 4 from Zoodsma et al., 2020
startle response increased occurrence, abnormal	grin1a^sbu90/sbu90	acoustic radiation	Fig. 10 from Zoodsma et al., 2020
habituation decreased efficacy, abnormal	grin1a^sbu90/sbu90	acoustic radiation	Fig. 10 from Zoodsma et al., 2020
larval feeding behavior disrupted, abnormal	grin1a^sbu92/sbu92	chemical treatment by environment: dizocilpine	text only from Zoodsma et al., 2020
eating behavior decreased occurrence, abnormal	grin1a^sbu92/sbu92	chemical treatment by environment: dizocilpine	text only from Zoodsma et al., 2020
swimming behavior occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 7, Fig. 9 from Zoodsma et al., 2020
eating behavior decreased occurrence, exacerbated	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 6 from Zoodsma et al., 2020
response to absence of light delayed, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	light duration	Fig. 6 from Zoodsma et al., 2020
swimming decreased duration, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	light duration	Fig. 8 from Zoodsma et al., 2020
whole organism viability, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 5 from Zoodsma et al., 2020
swimming increased duration, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 8 from Zoodsma et al., 2020
response to absence of light decreased occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 7 from Zoodsma et al., 2020
habituation decreased efficacy, exacerbated	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	acoustic radiation	Fig. 10 from Zoodsma et al., 2020
swimming increased linear velocity, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 8 from Zoodsma et al., 2020
larval feeding behavior disrupted, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 6 from Zoodsma et al., 2020
locomotory exploration behavior spatial distribution of a process, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 9 from Zoodsma et al., 2020
response to absence of light decreased occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	light duration	Fig. 6, Fig. 8 from Zoodsma et al., 2020
startle response increased occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	acoustic radiation	Fig. 10 from Zoodsma et al., 2020

Citations