CRISPR

CRISPR1-rnaset2

ID

ZDB-CRISPR-201117-4

Name

CRISPR1-rnaset2

Previous Names

None

Target

rnaset2 (1)

Sequence

5' - AAGATGGTTCTAGCAGATCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site is "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sh532	rnaset2

Expression

Gene expression in Wild Types + CRISPR1-rnaset2

No data available

Phenotype

Phenotype resulting from CRISPR1-rnaset2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-rnaset2

Phenotype	Fish	Conditions	Figures
brain isg15 expression increased amount, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 1 from Hamilton et al., 2020
microglial cell morphology, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 3 from Hamilton et al., 2020
whole organism swimming behavior process quality, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 5 from Hamilton et al., 2020
microglial cell apoptotic cell clearance decreased process quality, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 3 from Hamilton et al., 2020
brain ifnphi1 expression increased amount, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 1 from Hamilton et al., 2020
whole organism exploration behavior process quality, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 5 from Hamilton et al., 2020
brain il1b expression increased amount, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 1 from Hamilton et al., 2020
brain mxa expression increased amount, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 1 from Hamilton et al., 2020
whole organism decreased behavioural activity, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 5 from Hamilton et al., 2020
whole organism viability, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 5 from Hamilton et al., 2020
optic tectum apoptotic cell clearance decreased process quality, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 3 from Hamilton et al., 2020
brain inflammatory response increased occurrence, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. 1 from Hamilton et al., 2020
brain rnaset2 expression decreased amount, abnormal	rnaset2^sh532/sh532	standard conditions	Fig. S6 from Hamilton et al., 2020
microglial cell morphology, abnormal	rnaset2^sh532/sh532; sh378Tg	standard conditions	Fig. 2 from Hamilton et al., 2020
optic tectum microglial cell circular, abnormal	rnaset2^sh532/sh532; sh378Tg	standard conditions	Fig. 2 from Hamilton et al., 2020
optic tectum microglial cell has extra parts of type microglial cell vacuole, abnormal	rnaset2^sh532/sh532; sh378Tg	standard conditions	Fig. 2 from Hamilton et al., 2020

Citations