CRISPR

CRISPR3-nfatc1

ID

ZDB-CRISPR-201012-1

Name

CRISPR3-nfatc1

Previous Names

None

Target

Sequence

5' - TACGGCCCTGAGCTCGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bns276	nfatc1

Expression

Gene expression in Wild Types + CRISPR3-nfatc1

No data available

Phenotype

Phenotype resulting from CRISPR3-nfatc1

No data available

Phenotype of all Fish created by or utilizing CRISPR3-nfatc1

Phenotype	Fish	Conditions	Figures
heart nfatc1 expression decreased amount, abnormal	nfatc1^{bns276/bns276} (AB)	standard conditions	Fig. 5 from Gunawan et al., 2020
heart twist1b expression decreased amount, abnormal	nfatc1^{bns276/bns276} (AB)	standard conditions	Fig. 5 from Gunawan et al., 2020
atrioventricular canal twist1b expression absent, abnormal	nfatc1^{bns276/bns276} (AB)	standard conditions	Fig. 5 from Gunawan et al., 2020
atrioventricular valve closure, abnormal	nfatc1^{bns276/bns276} (AB)	standard conditions	Fig. 4 from Gunawan et al., 2020
atrium increased size, abnormal	nfatc1^{bns276/bns276} (AB)	standard conditions	Fig. 4 from Gunawan et al., 2020
heart blood circulation disrupted, abnormal	nfatc1^{bns276/bns276} (AB)	standard conditions	Fig. 4 from Gunawan et al., 2020
pericardium edematous, abnormal	nfatc1^{bns276/bns276} (AB)	standard conditions	Fig. 3 from Gunawan et al., 2020
heart valve interstitial cell GFP expression decreased distribution, abnormal	nfatc1^{bns276/bns276}; bns321Tg; nkuasrfp1aTg; ubs3Tg	standard conditions	Fig. 3 from Gunawan et al., 2020
heart valve interstitial cell cell proliferation involved in heart valve development decreased occurrence, abnormal	nfatc1^{bns276/bns276}; is4Tg	standard conditions	Fig. 3 from Gunawan et al., 2020
heart blood circulation disrupted, abnormal	nfatc1^bns276/+; twist1b^{bns353/bns353} (AB)	standard conditions	Fig. 5 from Gunawan et al., 2020

Citations