CRISPR

CRISPR1-sp7

ID

ZDB-CRISPR-200831-2

Name

CRISPR1-sp7

Previous Names

None

Target

sp7 (1)

Sequence

5' - TTCTGCCACACCTGGTAAAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3239	sp7

Expression

Gene expression in Wild Types + CRISPR1-sp7

No data available

Phenotype

Phenotype resulting from CRISPR1-sp7

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sp7

Phenotype	Fish	Conditions	Figures
whole organism spp1 expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
whole organism pthlha expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
whole organism acvr1l expression increased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
whole organism sparc expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
whole organism mag expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
whole organism bglap expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3, Fig. 5 from Chen et al., 2019
whole organism col10a1a expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
odontogenesis decreased occurrence, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 2 from Chen et al., 2019
whole organism col1a1a expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
whole organism crb1 expression increased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
whole organism dlx2b expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3, Fig. 5 from Chen et al., 2019
whole organism smoc2 expression decreased amount, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 3 from Chen et al., 2019
bone mineralization decreased occurrence, abnormal	sp7^{zf3239/zf3239} (AB)	standard conditions	Fig. 2 from Chen et al., 2019

Citations