CRISPR

CRISPR1-rhcgb

ID

ZDB-CRISPR-200708-2

Name

CRISPR1-rhcgb

Previous Names

None

Target

Sequence

5' - GGGCAACTGCTTCGGCTCCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ot202	rhcgb

Expression

Gene expression in Wild Types + CRISPR1-rhcgb

No data available

Phenotype

Phenotype resulting from CRISPR1-rhcgb

No data available

Phenotype of all Fish created by or utilizing CRISPR1-rhcgb

Phenotype	Fish	Conditions	Figures
whole organism slc9a3.2 expression increased amount, abnormal	rhcgb^ot202/ot202	low sodium	Fig. 4 from Zimmer et al., 2020
sodium ion import across plasma membrane increased occurrence, abnormal	rhcgb^ot202/ot202	low sodium	Fig. 2, Fig. 3 from Zimmer et al., 2020
gill filament rhcgb expression absent, abnormal	rhcgb^ot202/ot202	standard conditions	Fig. 1 from Zimmer et al., 2020
sodium ion import across plasma membrane increased occurrence, abnormal	rhcgb^ot202/ot202	control	Fig. 2, Fig. 3 from Zimmer et al., 2020
sodium ion import across plasma membrane increased occurrence, abnormal	rhcgb^ot202/ot202 + MO2-slc9a3.2	low sodium	Fig. 3 from Zimmer et al., 2020
sodium ion import across plasma membrane increased occurrence, abnormal	rhcgb^ot202/ot202 + MO2-slc9a3.2	control	Fig. 3 from Zimmer et al., 2020

Citations