CRISPR

CRISPR4-ar

ID
ZDB-CRISPR-200624-1
Name
CRISPR4-ar
Previous Names
None
Target
Sequence
5' - GGGTACCACTGCTCGTG - 3'
ZFIN BLAST NCBI BLAST Ensembl
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "CGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
sh516 ar
Expression
Gene expression in Wild Types + CRISPR4-ar
No data available
Phenotype
Phenotype resulting from CRISPR4-ar
No data available
Phenotype of all Fish created by or utilizing CRISPR4-ar
Phenotype Fish Conditions Figures
development of secondary sexual characteristics decreased occurrence, abnormal arsh516/sh516 control Figure S2 from Metzner et al., 2020
testis deformed, abnormal arsh516/sh516 control Figure S2 from Metzner et al., 2020
testis disorganized, abnormal arsh516/sh516 control Figure S2 from Metzner et al., 2020
caudal fin curved, exacerbated pkd2hu2173/hu2173; arsh516/sh516 chemical treatment by environment: androst-5-ene-3,17-dione Figure 2 with image from Metzner et al., 2020
caudal fin curved, abnormal pkd2hu2173/hu2173; arsh516/sh516 control Figure 2 with image from Metzner et al., 2020
Citations
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