CRISPR

CRISPR1-ssbp1

ID

ZDB-CRISPR-200615-2

Name

CRISPR1-ssbp1

Previous Names

None

Target

Sequence

5' - CCTCCACCTGCCTCATGACG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR1-ssbp1

Expressed Gene	Anatomy	Figures
mt-co1	whole organism	Fig. S6 from Del Dotto et al., 2019
mt-nd1	whole organism	Fig. S6 from Del Dotto et al., 2019

Phenotype

Phenotype resulting from CRISPR1-ssbp1

Phenotype	Fish	Figures
developmental growth decreased process quality, abnormal	WT + CRISPR1-ssbp1	text only from Del Dotto et al., 2019
lens mitochondrion decreased amount, abnormal	cms1Tg + CRISPR1-ssbp1	Fig. S6 from Del Dotto et al., 2019
lens mitochondrion EGFP expression decreased amount, abnormal	cms1Tg + CRISPR1-ssbp1	Fig. S6 from Del Dotto et al., 2019
whole organism viability, abnormal	WT + CRISPR1-ssbp1	text only from Del Dotto et al., 2019

Phenotype of all Fish created by or utilizing CRISPR1-ssbp1

Phenotype	Fish	Conditions	Figures
whole organism viability, abnormal	WT + CRISPR1-ssbp1	standard conditions	text only from Del Dotto et al., 2019
developmental growth decreased process quality, abnormal	WT + CRISPR1-ssbp1	standard conditions	text only from Del Dotto et al., 2019
lens mitochondrion decreased amount, abnormal	cms1Tg + CRISPR1-ssbp1	standard conditions	Fig. S6 from Del Dotto et al., 2019
lens mitochondrion EGFP expression decreased amount, abnormal	cms1Tg + CRISPR1-ssbp1	standard conditions	Fig. S6 from Del Dotto et al., 2019

Citations