CRISPR

CRISPR1-nkap

ID

ZDB-CRISPR-200512-9

Name

CRISPR1-nkap

Previous Names

None

Target

Sequence

5' - AGCCGATTTCTCCTCTTCTT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was GGG at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3216	nkap

Expression

Gene expression in Wild Types + CRISPR1-nkap

No data available

Phenotype

Phenotype resulting from CRISPR1-nkap

Phenotype	Fish	Figures
eye edematous, abnormal	AB + CRISPR1-nkap	Fig. S5 from Fiordaliso et al., 2019
gut edematous, abnormal	AB + CRISPR1-nkap	Fig. S5 from Fiordaliso et al., 2019
heart edematous, abnormal	AB + CRISPR1-nkap	Fig. S5 from Fiordaliso et al., 2019
notochord deformed, abnormal	AB + CRISPR1-nkap	Fig. S5 from Fiordaliso et al., 2019

Phenotype of all Fish created by or utilizing CRISPR1-nkap

Phenotype	Fish	Conditions	Figures
whole organism decreased life span, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
embryo development disrupted, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
central nervous system necrotic, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
heart edematous, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
notochord curved, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
heart contraction disrupted, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
gut edematous, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
eye edematous, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
whole organism dead, abnormal	nkap^{zf3216/zf3216} (AB)	standard conditions	Fig. 5 from Fiordaliso et al., 2019
heart edematous, abnormal	AB + CRISPR1-nkap	standard conditions	Fig. S5 from Fiordaliso et al., 2019
notochord deformed, abnormal	AB + CRISPR1-nkap	standard conditions	Fig. S5 from Fiordaliso et al., 2019
gut edematous, abnormal	AB + CRISPR1-nkap	standard conditions	Fig. S5 from Fiordaliso et al., 2019
eye edematous, abnormal	AB + CRISPR1-nkap	standard conditions	Fig. S5 from Fiordaliso et al., 2019

Citations