CRISPR

CRISPR2-six1a

ID
ZDB-CRISPR-191024-9
Name
CRISPR2-six1a
Previous Names
None
Target
Sequence
5' - GGCGCACCCAGGCCGGGCAT - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
Df(Chr13:six1a,six4a)oz27 six1a, six4a
Expression
Gene expression in Wild Types + CRISPR2-six1a
No data available
Phenotype
Phenotype resulting from CRISPR2-six1a
No data available
Phenotype of all Fish created by or utilizing CRISPR2-six1a
Phenotype Fish Conditions Figures
migratory muscle precursor cell migration delayed, abnormal Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
middle migratory muscle precursor stream met expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S4 with image from Talbot et al., 2019
posterior migratory muscle precursor stream met expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S4 with image from Talbot et al., 2019
whole organism lacks parts or has fewer parts of type otic epithelium, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S3 with image from Talbot et al., 2019
posterior lateral line absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S3 with image from Talbot et al., 2019
anterior migratory muscle precursor stream met expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S4 with image from Talbot et al., 2019
anterior migratory muscle precursor stream lbx1a expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S4 with image from Talbot et al., 2019
dorsal-ventral polarized posterior lateral line neuromast position, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S3 with image from Talbot et al., 2019
middle migratory muscle precursor stream lbx1a expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S4 with image from Talbot et al., 2019
posterior migratory muscle precursor stream lbx1a expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S4 with image from Talbot et al., 2019
otic vesicle decreased size, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S3 with image from Talbot et al., 2019
anterior lateral line neuromast position, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+ standard conditions Fig. S3 with image from Talbot et al., 2019
otic epithelium absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 2 with image from Talbot et al., 2019
whole organism lacks parts or has fewer parts of type primitive pectoral fin adductor, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 2 with image from Talbot et al., 2019
whole organism lacks parts or has fewer parts of type primitive pectoral fin abductor, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 2 with image from Talbot et al., 2019
posterior hypaxial muscle decreased size, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 2 with image from Talbot et al., 2019
posterior hypaxial muscle position, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 2 with image from Talbot et al., 2019
primary posterior lateral line primordium absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 2 with image from Talbot et al., 2019
primary posterior lateral line primordium GFP expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 3 with image from Talbot et al., 2019
otic vesicle decreased size, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Fig. 2 with image from Talbot et al., 2019
primitive pectoral fin abductor decreased size, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
middle migratory muscle precursor stream hypoplastic, exacerbated Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
anterior migratory muscle precursor stream hypoplastic, exacerbated Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
posterior hypaxial muscle decreased size, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
posterior migratory muscle precursor stream hypoplastic, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
migratory muscle precursor cell migration delayed, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
posterior migratory muscle precursor stream hypoplastic, exacerbated Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
primitive pectoral fin adductor decreased size, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
somite ventral-most region met expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; cz3327Tg; oz5Tg standard conditions Fig. 3 with image from Talbot et al., 2019
somite 2 ventral-most region lbx1a expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; cz3327Tg; oz5Tg standard conditions Fig. 3 with image from Talbot et al., 2019
somite 1 ventral-most region lbx1a expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; cz3327Tg; oz5Tg standard conditions Fig. 3 with image from Talbot et al., 2019
somite 3 ventral-most region lbx1a expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; cz3327Tg; oz5Tg standard conditions Fig. 3 with image from Talbot et al., 2019
primary posterior lateral line primordium met expression absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; cz3327Tg; oz5Tg standard conditions Fig. 3 with image from Talbot et al., 2019
migratory muscle precursor cell fate commitment disrupted, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; cz3327Tg; oz5Tg standard conditions Fig. 3 with image from Talbot et al., 2019
whole organism lacks parts or has fewer parts of type primitive pectoral fin adductor, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
whole organism lacks parts or has fewer parts of type primitive pectoral fin abductor, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
posterior migratory muscle precursor stream hypoplastic, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
whole organism lacks parts or has fewer parts of type posterior hypaxial muscle muscle cell, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
anterior migratory muscle precursor stream absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
sternohyoid absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
posterior migratory muscle precursor stream hypoplastic, exacerbated Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
migratory muscle precursor cell migration delayed, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
middle migratory muscle precursor stream absent, abnormal Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/oz27; oz29Tg; oz5Tg standard conditions Fig. 5 with image from Talbot et al., 2019
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