CRISPR

CRISPR1-dram1

ID

ZDB-CRISPR-190711-1

Name

CRISPR1-dram1

Previous Names

None

Target

dram1 (1)

Sequence

5' - GACCAGATAACCAGGAAAGTT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ibl53	dram1

Expression

Gene expression in Wild Types + CRISPR1-dram1

No data available

Phenotype

Phenotype resulting from CRISPR1-dram1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-dram1

Phenotype	Fish	Conditions	Figures
defense response to bacterium decreased efficacy, abnormal	dram1^ibl53/ibl53	bacterial treatment by injection: Mycobacterium marinum	Figure 3 from Xie et al., 2024
defense response to bacterium decreased efficacy, abnormal	dram1^ibl53/ibl53	bacterial treatment by injection: Mycobacterium marinum M	Fig. S3 from Sommer et al., 2019
macrophage phagocytic vesicle increased amount, exacerbated	dram1^ibl53/ibl53 (AB/TL)	chemical treatment by environment: bafilomycin A1	Fig. 3 from Zhang et al., 2020
defense response to bacterium disrupted, abnormal	dram1^ibl53/ibl53 (AB/TL)	standard conditions	Fig. 1 from Zhang et al., 2020
defense response to bacterium decreased efficacy, abnormal	dram1^ibl53/ibl53 (AB/TL)	bacterial treatment by injection: Mycobacterium marinum	Fig. 6 from Zhang et al., 2020
whole organism map1lc3b expression increased amount, abnormal	dram1^ibl53/ibl53 (AB/TL)	bacterial treatment by injection: Mycobacterium marinum	Fig. 3 from Zhang et al., 2020
whole organism ab4-sqstm1 labeling increased amount, abnormal	dram1^ibl53/ibl53 (AB/TL)	chemical treatment by environment: bafilomycin A1	Fig. 3 from Zhang et al., 2020
macrophage pyroptotic inflammatory response increased occurrence, abnormal	dram1^ibl53/ibl53 (AB/TL)	bacterial treatment by injection: Mycobacterium marinum	Fig. 6 from Zhang et al., 2020
whole organism map1lc3b expression increased amount, abnormal	dram1^ibl53/ibl53 (AB/TL)	chemical treatment by environment: bafilomycin A1	Fig. 3 from Zhang et al., 2020
whole organism ab2-optn labeling increased amount, abnormal	dram1^ibl53/ibl53 (AB/TL)	chemical treatment by environment: bafilomycin A1	Fig. 3 from Zhang et al., 2020
macrophage phagocytosis decreased rate of occurrence, abnormal	dram1^ibl53/ibl53; ump2Tg	bacterial treatment by injection: Mycobacterium marinum	Fig. 5 from Zhang et al., 2020
defense response to bacterium disrupted, abnormal	dram1^ibl53/ibl53; ump2Tg	bacterial treatment by injection: Mycobacterium marinum	Fig. 5 from Zhang et al., 2020
defense response to bacterium decreased efficacy, ameliorated	dram1^ibl53/ibl53 + MO1-caspa (AB/TL)	bacterial treatment by injection: Mycobacterium marinum	Fig. 6 from Zhang et al., 2020
defense response to bacterium decreased efficacy, ameliorated	dram1^ibl53/ibl53 + MO4-gsdmeb (AB/TL)	bacterial treatment by injection: Mycobacterium marinum	Fig. 6 from Zhang et al., 2020
macrophage migration disrupted, abnormal	dram1^ibl53/ibl53; zf155Tg	bacterial treatment by injection: Mycobacterium marinum	Fig. 3 from Zhang et al., 2020

Citations