CRISPR

CRISPR1-myo1d

ID

ZDB-CRISPR-190211-1

Name

CRISPR1-myo1d

Previous Names

None

Target

myo1d (1)

Sequence

5' - GAGTGGAGCTGGAAAAACAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
nce16b	myo1d
nce16c	myo1d

Expression

Gene expression in Wild Types + CRISPR1-myo1d

No data available

Phenotype

Phenotype resulting from CRISPR1-myo1d

No data available

Phenotype of all Fish created by or utilizing CRISPR1-myo1d

Phenotype	Fish	Conditions	Figures
epithalamus dorsal side pitx2 expression decreased amount, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
epithalamus dorsal side pitx2 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
Kupffer's vesicle decreased area, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 3 from Juan et al., 2018
Kupffer's vesicle cilium posterior orientation, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 6 from Juan et al., 2018
liver foxa1 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
Kupffer's vesicle area, ameliorated	myo1d^{nce16b/nce16b}	chemical treatment by environment: forskolin, chemical treatment by environment: 3-isobutyl-1-methylxanthine	Fig. 3 from Juan et al., 2018
Kupffer's vesicle ciliary basal body mislocalised anteriorly, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 6 from Juan et al., 2018
heart heart looping decreased process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
determination of pancreatic left/right asymmetry process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
determination of heart left/right asymmetry decreased process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
heart jogging decreased process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
Kupffer's vesicle dand5 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 2 from Juan et al., 2018
gut foxa1 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
pancreas foxa1 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
gut determination of digestive tract left/right asymmetry process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
heart tube heart jogging decreased process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
heart mislocalised, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
determination of left/right asymmetry in nervous system process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. S2 from Juan et al., 2018
determination of liver left/right asymmetry process quality, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
heart tube mislocalised, abnormal	myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Juan et al., 2018
pancreas left/right pattern formation decreased process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
heart tube mislocalised, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Juan et al., 2018
Kupffer's vesicle decreased area, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 5 from Juan et al., 2018
lateral plate mesoderm left side spaw expression spatial pattern, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 2 from Kurup et al., 2024
Kupffer's vesicle epithelial cilium movement involved in determination of left/right asymmetry process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 4 from Juan et al., 2018
brain left/right pattern formation decreased process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
gut left/right pattern formation decreased process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
determination of heart left/right asymmetry decreased process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Juan et al., 2018
heart heart looping decreased process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Juan et al., 2018
heart jogging decreased process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
Kupffer's vesicle cilium decreased size, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 5 from Juan et al., 2018
heart tube heart jogging decreased process quality, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Juan et al., 2018
heart mislocalised, abnormal	myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Juan et al., 2018
pancreas left/right pattern formation decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
lateral plate mesoderm left side spaw expression spatial pattern, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}	standard conditions	Fig. 3 from Kurup et al., 2024
brain left/right pattern formation decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
shield maintenance of ciliary planar beating movement pattern decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
lateral plate mesoderm left side spaw expression decreased amount, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}	standard conditions	Fig. 3 from Kurup et al., 2024
gut left/right pattern formation decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
heart jogging decreased process quality, exacerbated	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}	standard conditions	Fig. 1 from Kurup et al., 2024
epithalamus dorsal side pitx2 expression decreased amount, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
determination of heart left/right asymmetry decreased process quality, exacerbated	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Juan et al., 2018
epithalamus dorsal side pitx2 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
lateral plate mesoderm left side spaw expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 2 from Kurup et al., 2024
Kupffer's vesicle epithelial cilium movement involved in determination of left/right asymmetry process quality, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 4 from Juan et al., 2018
brain left/right pattern formation decreased process quality, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
lateral plate mesoderm left side pitx2 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 2 from Kurup et al., 2024
heart tube mislocalised, exacerbated	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Juan et al., 2018
liver foxa1 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
heart mislocalised, exacerbated	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Juan et al., 2018
heart heart looping decreased process quality, exacerbated	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Juan et al., 2018
Kupffer's vesicle dand5 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 2 from Juan et al., 2018
gut foxa1 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
pancreas foxa1 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
heart tube heart jogging decreased process quality, exacerbated	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Juan et al., 2018
epithalamus left/right pattern formation decreased process quality, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
notochord lft1 expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 2 from Kurup et al., 2024
heart jogging decreased process quality, exacerbated	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
Kupffer's vesicle decreased area, abnormal	myo1d^{nce16b/nce16b}; vangl2^m209/+	standard conditions	Fig. 5 from Juan et al., 2018
Kupffer's vesicle cilium decreased size, abnormal	myo1d^{nce16b/nce16b}; vangl2^m209/+	standard conditions	Fig. 5 from Juan et al., 2018
Kupffer's vesicle epithelial cilium movement involved in determination of left/right asymmetry process quality, ameliorated	myo1d^{nce16b/nce16b}; vangl2^m209/+	standard conditions	Fig. 4 from Juan et al., 2018
Kupffer's vesicle ciliary basal body mislocalised anteriorly, abnormal	myo1d^{nce16b/nce16b}; vangl2^m209/+	standard conditions	Fig. 6 from Juan et al., 2018
Kupffer's vesicle cilium position, ameliorated	myo1d^{nce16b/nce16b}; vangl2^m209/+	standard conditions	Fig. 6 from Juan et al., 2018
pancreas left/right pattern formation decreased process quality, abnormal	myo1g^{nce18b/nce18b}; myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
brain left/right pattern formation decreased process quality, abnormal	myo1g^{nce18b/nce18b}; myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
gut left/right pattern formation decreased process quality, abnormal	myo1g^{nce18b/nce18b}; myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
heart jogging decreased process quality, exacerbated	myo1g^{nce18b/nce18b}; myo1d^{nce16c/nce16c}	standard conditions	Fig. 1 from Kurup et al., 2024
heart primordium lft2 expression decreased amount, abnormal	myo1g^{nce18b/nce18b}; myo1d^{nce16c/nce16c}	standard conditions	Fig. 2 from Kurup et al., 2024
heart primordium lft2 expression spatial pattern, abnormal	myo1g^{nce18b/nce18b}; myo1d^{nce16c/nce16c}	standard conditions	Fig. 2 from Kurup et al., 2024
pancreas left/right pattern formation decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
lateral plate mesoderm left side spaw expression spatial pattern, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 3 from Kurup et al., 2024
shield maintenance of ciliary planar beating movement pattern decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
brain left/right pattern formation decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
lateral plate mesoderm left side spaw expression decreased amount, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 3 from Kurup et al., 2024
liver foxa1 expression spatial pattern, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
gut left/right pattern formation decreased process quality, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
pancreas foxa1 expression spatial pattern, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
gut foxa1 expression spatial pattern, abnormal	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
heart jogging decreased process quality, exacerbated	dnaaf1^{tm317b/tm317b}; myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b}	standard conditions	Fig. 1 from Kurup et al., 2024
lateral plate mesoderm left side spaw expression spatial pattern, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b} + MO1-dnaaf1	standard conditions	Fig. 3 from Kurup et al., 2024
lateral plate mesoderm left side spaw expression decreased amount, abnormal	myo1d^{nce16b/nce16b}; myo1g^{nce18b/nce18b} + MO1-dnaaf1	standard conditions	Fig. 3 from Kurup et al., 2024

Citations