CRISPR

CRISPR1-g6pd

ID

ZDB-CRISPR-190115-7

Name

CRISPR1-g6pd

Previous Names

None

Target

g6pd (1)

Sequence

5' - GAGAAGGGGAGGCAAAACTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The gene-specific oligo sequence was 5′-TAATACGACTCACTATAGAGAAGGGGAGGCAAAACTGGTTTTAGAGCTAG AAATAGCAAG-3'.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR1-g6pd

No data available

Phenotype

Phenotype resulting from CRISPR1-g6pd

Phenotype	Fish	Figures
glucose-6-phosphate dehydrogenase activity decreased occurrence, abnormal	AB + CRISPR1-g6pd	Fig. 4 from Hwang et al., 2018
pericardium edematous, abnormal	AB + CRISPR1-g6pd	Fig. 4 from Hwang et al., 2018
whole organism NADPH decreased amount, abnormal	AB + CRISPR1-g6pd	Fig. 4 from Hwang et al., 2018
whole organism reactive oxygen species increased amount, abnormal	AB + CRISPR1-g6pd	Fig. 4 from Hwang et al., 2018

Phenotype of all Fish created by or utilizing CRISPR1-g6pd

Phenotype	Fish	Conditions	Figures
blood accumulation pericardial region, abnormal	AB + CRISPR1-g6pd	chemical treatment: chloroquine, chemical treatment: N,N'-[disulfanediyldi(ethane-2,1-diyl)]bis[2-(1H-indol-3-yl)ethan-1-amine]	Fig. 4 from Hwang et al., 2018
whole organism reactive oxygen species increased amount, exacerbated	AB + CRISPR1-g6pd	chemical treatment: chloroquine	Fig. 4 from Hwang et al., 2018
glucose-6-phosphate dehydrogenase activity decreased occurrence, abnormal	AB + CRISPR1-g6pd	chemical treatment: N,N'-[disulfanediyldi(ethane-2,1-diyl)]bis[2-(1H-indol-3-yl)ethan-1-amine]	Fig. 4 from Hwang et al., 2018
whole organism reactive oxygen species increased amount, exacerbated	AB + CRISPR1-g6pd	chemical treatment: chloroquine, chemical treatment: N,N'-[disulfanediyldi(ethane-2,1-diyl)]bis[2-(1H-indol-3-yl)ethan-1-amine]	Fig. 4 from Hwang et al., 2018
pericardium edematous, abnormal	AB + CRISPR1-g6pd	control	Fig. 4 from Hwang et al., 2018
whole organism NADPH decreased amount, abnormal	AB + CRISPR1-g6pd	control	Fig. 4 from Hwang et al., 2018
glucose-6-phosphate dehydrogenase activity decreased occurrence, abnormal	AB + CRISPR1-g6pd	chemical treatment: chloroquine	Fig. 4 from Hwang et al., 2018
glucose-6-phosphate dehydrogenase activity decreased occurrence, abnormal	AB + CRISPR1-g6pd	control	Fig. 4 from Hwang et al., 2018
whole organism reactive oxygen species increased amount, abnormal	AB + CRISPR1-g6pd	control	Fig. 4 from Hwang et al., 2018
pericardium edematous, abnormal	AB + CRISPR1-g6pd	chemical treatment: chloroquine, chemical treatment: N,N'-[disulfanediyldi(ethane-2,1-diyl)]bis[2-(1H-indol-3-yl)ethan-1-amine]	Fig. 4 from Hwang et al., 2018
pericardium edematous, exacerbated	AB + CRISPR1-g6pd	chemical treatment: chloroquine	Fig. 4 from Hwang et al., 2018

Citations